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Fig. 6 | Mobile DNA

Fig. 6

From: Transposable Element (TE) insertion predictions from RNAseq inputs and TE impact on RNA splicing and gene expression in Drosophila brain transcriptomes

Fig. 6

Testing the effect of a TE insertion within an intron on host gene mRNA splicing and expression. Diagrams showing the w1118 strain has blood TE insertion in Dscam2 gene (A-i) and opus TE insertion in Bx gene (B-i), while the OreR strain does not have those TE insertions in the corresponding genes. (A-ii) RT-qPCR assay examining spliced Dscam2 mRNA levels in the heads of w1118 and OreR. (A-iii) ddPCR assay replicating the RT-qPCR result in (A-ii). (B-ii) RT-qPCR assay examining spliced Bx mRNA levels in the heads of w1118 and OreR. T-tests p-values: * = p < 0.05, ** = p < 0.005, *** = p < 0.001

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Mobile DNA

ISSN: 1759-8753