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Fig. 5 | Mobile DNA

Fig. 5

From: Transposable Element (TE) insertion predictions from RNAseq inputs and TE impact on RNA splicing and gene expression in Drosophila brain transcriptomes

Fig. 5

Rigorous gDNA-PCR validation of homozygous TE insertions in selected genes in the Drosophila w1118 strain genome. Panels correspond to the TE-gene pairs (A) pogo-CaMKII, (B) I-element-Pde1C, (C) opus-Bx, and (D) blood-Dscam2. Each figure panel is divided in parts (i) that is a diagram of the presumptive TE-gene splicing event proposed by Treiber and Waddell 2020, (ii) UCSC Genome Browser snapshots of the example split reads support for the TE insertion from TIDAL analysis of the w1118 midbrain RNAseq data, (iii) gel images of gDNA-PCR amplicons (left set) and RT-PCR amplicons (right set) from the various sets of primer pairs illustrated in the diagram above the gel images. Solid lines around gels indicates cropped gel images; dashed lines represents different sections on a single gel

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ISSN: 1759-8753