Fig. 3
From: Internal initiation of reverse transcription in a Penelope-like retrotransposon
Structure and cleavage properties of the PAc hammerhead ribozyme (HHR). A The HHR encoded by PAc is conserved across other Anolis species. Stem I, stem II, and loop II from the canonical HHR structure along with the HHR cut site and PAc start codon are annotated. pLTR, pseudo long terminal repeat; ORF, open reading frame. Visualization of the PAc HHR secondary structure is shown next to the sequence alignment. Residues C3 and G8 in the HHR catalytic core are boxed. B Visualizing the role of the HHR and GIY-YIG endonuclease in RNA processing and extension in vitro. G8C and C3G are HHR mutants while G8C + C3G is a partial rescue. G19A and Y17A are key mutations in the active site of the GIY-YIG endonuclease